Polymerase chain reaction in rapid diagnosis of neonatal sepsis.
Indian Pediatr
;
2005 Jul; 42(7): 681-5
Article
in English
| IMSEAR
| ID: sea-15034
ABSTRACT
In a prospective study a total of hundred neonates who fulfilled the American College of Obstetrics and Gynecology's (ACOG) criteria for probable sepsis admitted to NICU of tertiary care armed forces hospital were investigated for evidence of sepsis. The investigation protocol included sepsis screen, blood culture and 1 mL of venous blood for molecular analysis by polymerase chain reaction (PCR) for bacterial DNA component encoding 16 s RNA in all cases. 100 newborns with probable sepsis were studied to evaluate the molecular diagnosis of sepsis using PCR amplification of 16 S RNA in newborns with risk factors for sepsis or those who have clinical evidence of sepsis. We compared the results of PCR with blood culture and other markers of sepsis screen (total leucocyte count (TLC), absolute neutrophil count (ANC), immature/total neutrophil count ratio (I/T ratio), peripheral blood smear, micro ESR and C reactive protein (CRP). Controls consisted of 30 normal healthy newborns with no overt evidence of sepsis. Sepsis screen was positive in 24 (24%) of cases in study group with sensitivity and specificity of 100% and 83.5% respectively. Blood culture was positive in 09(9%t) with sensitivity of 69.2% and specificity of 100%. PCR was positive in 13(13%) of cases (9% are both blood culture and sepsis screen positive and 4% are positive by sepsis screen); the sensitivity of PCR was 100% and specificity was 95.6%. Blood culture is the most reliable method for diagnosis of neonatal sepsis. Polymerase chain reaction is useful and superior to blood culture for early diagnosis of sepsis in neonates.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Bacterial Infections
/
Blood Cell Count
/
Blood Sedimentation
/
Humans
/
C-Reactive Protein
/
DNA, Bacterial
/
Infant, Newborn
/
RNA, Ribosomal, 16S
/
Polymerase Chain Reaction
/
Predictive Value of Tests
Type of study:
Practice guideline
/
Observational study
/
Prognostic study
/
Risk factors
/
Screening study
Language:
English
Journal:
Indian Pediatr
Year:
2005
Type:
Article
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