Investigation on ultrasound-assisted extraction of three dibenzylbutyrolactone lignans from Hemistepta lyrata.

ABSTRACT

Ultrasound-assisted extraction is evaluated as a simpler and more effective alternative to conventional extraction methods for the extraction of bioactive compounds from natural product. This study investigated the use of ultrasound-assisted extraction to extract three dibenzylbutyrolactone lignans, including tracheloside, hemislienoside, and arctiin from Hemistepta lyrata. Factors such as extraction solvent, solvent concentration, solvent to material ratio, and extraction time were examined. High-performance liquid chromatography with photodiode array detection was used for simultaneous determination of the target compounds in the corresponding extracts. Results showed that the optimal parameters to extract the target compounds from H. lyrata were as follows extraction solvent 70% aqueous ethanol; solvent to material ratio 201 (v/w, ml/g); extraction time 20 min under the conditions ultrasonic frequency 40 Hz; extraction temperature 30 ˚C. With all these merits, ultrasound-assisted extraction should be considered for wider application in the extraction of tracheloside, hemislienoside, and arctiin from other medicinal plants.