Evaluation of Hepatoprotective and antioxidant potential of methanolic extract of Polyalthiya longifolia fruits: An In-vitro and In-vivo approach.

ABSTRACT

The aim of the study was to investigate the hepatoprotective and antioxidant potential of methanolic extract of Polyalthiya longifolia fruits (MEPL) by in-vitro and in-vivo methods.In the invitro study, Freshly isolated rat primary hepatocytes and HepG2 cells were exposed with CCl4 along with/without various concentrations of MEPL(125,250,500 μg/kg) and the effects were studied.In the in-vivo studies, CCl4 intoxication method was used and aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total bilirubin and total proteins were estimated and supported with histopathological studies.MEPL (125,250,500 μg/kg) treated animals increased the percentage of viability in both primary hepatocytes (p<0.001) and in HepG2 cells (p<0.01) where as in the invivo studies,MEPL produced significant hepatoprotective effect by decreasing the elevated serum enzymes, bilirubin, LPO and significantly increased the levels of glutathione (GSH), catalase (CAT) and super oxide dismutase (SOD) .Moreover, in-vitro antioxidant studies revealed that MEPL scavenged free radicals and maximum percentage of inhibition was 62% at 800μg/mL. In addition to this, treatment with MEPL fruits showed a dose dependent reversal of histopathological changes. Based on the results, we conclude that, MEPL possesses hepatoprotective and antioxidant activity against CCl4 intoxication in both in-vitro and in-vivo methods through its free radical scavenging and antioxidant properties.