Comparison of alternate glycolysis inhibitors with fluoride for preservation of blood for glucose and other common clinical chemistry examinations.

ABSTRACT

Aims: The study aims at comparing observed values of glucose and various common clinical chemistry examinations in sample containers with different glycolysis inhibitor – Glyceraldehyde. Materials And Methods: Prepare sample conainers with alternate glycolysis inhibitors.Collect blood samples in prepared sample containers (systematic sampling of 100 consenting patients admitted at various wards of New civil Hospital,Surat for each perservative).Observe values of various examinations in collected samples. Statistical Analysis Used Perform two-sample Student's t-test on observed sets of data. Results: Average Glucose concentration in 5 mmol/L DL-Glyceraldehyde, Fluoride and Plain tube each was 118.78 mg/dl, 121.84 mg/dl and 100.24 mg/dl respectively after 8 hours. P-value for glyceraldehyde tube vs. Fluoride tube was 0.64737, P-value for glyceraldehyde tube vs. Plain tube was 0.00796 and P-value for Fluoride tube vs. Plain tube was 0.00187. P-value for Bilirubin, ALT,ALP, Amylase,Electrolytes, Triglycerides, Cholesterol, HDL Cholesterol, Urea, Uric Acid, Albumin, Total Proteins were unaffected by DL-Glyceraldehyde, while there was negative interferance with Creatinine measurement by Alkaline Picrate method. Conclusions: Use of DL-Glyceraldehyde containers for preservation of blood for Glucose measurement is as good as fluoride containers. Because Glyceraldehyde interfere with creatinine assay by Alkaline Picrate method and pottasium assay by ISE , Glyceraldehyde containers can not replace Plain tube for common routine clinical chemistry analysis.