Comparative evaluation of the antimicrobial activity of different endodontic sealers on enterococcus faecalis – an invitro study.

ABSTRACT

Background and objectives: To evaluate the in vitro antimicrobial activity of different root canal sealers. Materials and method: Five root canal sealers were selected for the study namely Zinc Oxide Eugenol, Endoflas FS, Endomethasone, AH plus and Sealapex . Enterococcus faecalis was obtained from American Type Culture Collection (ATCC 29212).The media used were Brain Heart Infusion Broth (BHI) and Blood Agar. 10 blood agar plates of 15 x 100 mm were prepared each inoculated with prepared E. faecalis to obtain Lawn culture. Ampicillin discs (10g) were used as the control discs. The sealers were manipulated under the UV laminar flow chamber. 100 microlitre (0.1 ml) of each sealer was placed on the sterile paper disc with micropipettes. The zones of inhibition were measured at 24 hours and 48 hours from the edge of the paper discs with help of vernier calipers and recorded. Results: Kruskal-wallis one way ANOVA was used to calculate the overall P-value. Mann – Whitney U-test was employed to identify the significant groups at 5% level after correcting the p-values for multiple comparisons by Bonferroni correction method. Conclusion: Endomethasone showed significantly greater antimicrobial effect against E. faecalis. There was no significant difference of antimicrobial activity between Zinc Oxide Eugenol and Endoflas FS on E. faecalis. Sealapex was less effective against E .feacalis. AH plus showed no antimicrobial activity on E. faecalis.