In vitro regeneration of Coelogyne nervosa A.Rich. and Eria pseudoclavicaulis Blatt., threatened orchids of Western Ghats, India.
Indian J Exp Biol
;
2014 Jun; 52(6): 658-663
Article
in English
| IMSEAR
| ID: sea-153745
ABSTRACT
The seeds of C. nervosa and E. pseudoclavicaulis were germinated asymbiotically on Knudson C (KC) and Schenk and Hildebrandt basal medium (SH). Growth regulators such as 2,4-Dichlorophenoxyacetic acid (2,4-D) individually and in combinations with benzyladenine (BA) and kinetin were used for callus induction from the protocorm like bodies. Coelogyne nervosa showed maximum (90%) callus induction in Knudson C medium supplemented with 2,4-D (2.26 µM) and Eria pseudoclavicaulis showed 60% callus induction in Schenk and Hildebrandt medium supplemented with 2,4-D (2.26 µM). Calli developed a route of production of protocorm-like bodies and eventually developed into plantlets on transfer to growth regulator free half strength basal medium. The well rooted plants were hardened successfully in the potting mixture containing coconut husk, charcoal, and brick pieces in the ratio 211.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Plant Growth Regulators
/
Regeneration
/
Cells, Cultured
/
Plant Roots
/
Endangered Species
/
Cell Culture Techniques
/
Culture Media
/
Orchidaceae
/
2,4-Dichlorophenoxyacetic Acid
/
India
Country/Region as subject:
Asia
Language:
English
Journal:
Indian J Exp Biol
Year:
2014
Type:
Article
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