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Analysis on the arcelin expression in bruchid pest resistant wild pulses using real time RT-qPCR.
Indian J Exp Biol ; 2014 Dec; 52(12): 1195-1200
Article in English | IMSEAR | ID: sea-153811
ABSTRACT
Arcelin, the antimetabolic protein from wild pulses is a known natural insecticidal molecule. Wild pulses with high arcelin content could serve as potential source to increase the levels of insect resistance in cultivated pulse crops. In this study, arcelin (Arl) gene expression was screened in seven stored product insect pest resistant wild pulse varieties using real time RT-qPCR. Arcelin gene specific real time PCR primers were synthesized from arcelin mRNA sequence of the wild pulse variety, Lablab purpureus. The results revealed different levels of arcelin gene expression in the tested varieties. Canavalia virosa registered significantly high content indicating its suitability for utilization of arcelin gene in developing stored product insect pest resistance with other cultivated pulses.
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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Plant Diseases / Seeds / Species Specificity / Coleoptera / Glycoproteins / Gene Expression Regulation, Plant / Reverse Transcriptase Polymerase Chain Reaction / Phaseolus / Canavalia / Disease Resistance Language: English Journal: Indian J Exp Biol Year: 2014 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Plant Diseases / Seeds / Species Specificity / Coleoptera / Glycoproteins / Gene Expression Regulation, Plant / Reverse Transcriptase Polymerase Chain Reaction / Phaseolus / Canavalia / Disease Resistance Language: English Journal: Indian J Exp Biol Year: 2014 Type: Article