Your browser doesn't support javascript.
loading
Purification and characterization of structural and functional properties of two lectins from a marine sponge Spheciospongia vesparia.
Indian J Biochem Biophys ; 2013 Dec; 50(6): 562-569
Article in English | IMSEAR | ID: sea-154215
ABSTRACT
The purification, structural and functional characterization of two different lectins (named Svl-1 and Svl-2) has been reported from the marine sponge Spheciospongia vesparia. Purification procedure includes ammonium sulfate precipitation, combined with chromatography including Octyl-Sepharose-(NH4)SO4 hydrophobic column and DEAE-Toyopearl anion-exchange column using a high performance liquid chromatography. The similarities in function, specificity for saccharides, molecular weight, amino acid content and the N-terminal sequence of two lectins suggest that these proteins are isolectins. Amino acid composition and fluorescence analyses reveal that they contain an intrachain disulfide bridge, which might contribute to their high thermal stability. Furthermore, the purified lectins exhibit antibacterial activity against the gram-negative bacteria Pseudomonas aeruginosa and E. coli, indicating that they may be involved in a recognition strategy and may play a role in the defense response function of the sponge. This is the first report on the isolation of lectins from the S. vesparia. The purified lectins represent a potential possible candidate for future application in the recognition or treatment of cancer cells.
Subject(s)

Full text: Available Index: IMSEAR (South-East Asia) Main subject: Porifera / Bacteria / Humans / Sequence Homology / Sequence Analysis / Aquatic Organisms / Hemagglutination / Lectins / Animals / Anti-Bacterial Agents Language: English Journal: Indian J Biochem Biophys Year: 2013 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: IMSEAR (South-East Asia) Main subject: Porifera / Bacteria / Humans / Sequence Homology / Sequence Analysis / Aquatic Organisms / Hemagglutination / Lectins / Animals / Anti-Bacterial Agents Language: English Journal: Indian J Biochem Biophys Year: 2013 Type: Article