Comparison of immunofluorescence and enzyme-linked immunosorbent assay and immunoglobulin G avidity techniques for screening of anti: Toxoplasma antibodies among single serum sample pregnant women in Tabriz, Iran.
Article
in English
| IMSEAR
| ID: sea-156180
ABSTRACT
Background:
Congenital toxoplasmosis is that pregnant women acquire the infection during gestation; diagnosis of the acute infection during pregnancy is a complex subject of maternal toxoplasmosis. Thus, the presence of immunoglobulin G (IgG) and/or IgM Toxoplasma antibodies in a single serum sample drawn during gestation cannot be used to defi ne whether the infection was recently acquired or chronic. Materials andMethods:
At this cross-sectional descriptive study, sera of 391 pregnant women examined and compared. They were in an age range of 21-35 years, referred by gynecologists and infectious disease specialists, during March 2012-April 2013. They have referred, 215 (54.98%), 102 (26%), 74 (18.92%) in the fi rst, second and third trimesters of gestation, respectively. For each of them, a questionnaire was completed and serum samples were prepared in an equal condition, examined according to the procedures of indirect immunofl uorescence (IIF), enzyme-linked immunosorbent assay (ELISA) and IgG Avidity techniques.Results:
We have found 111 (28.38%) seronegative and 280 (71.61%) seropositive cases by IIF and 124 (31.70%) seronegative, 267 (68.28%) seropositive cases by ELISA. The IgG avidity test confi rmed 45 (69.23%) and 7 (10.76%) doubtful cases of IgM test in IIF and ELISA techniques.Conclusions:
This study highlights how to manage pregnant women with toxoplasmosis, especially in a single serum sample condition.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Type of study:
Diagnostic study
/
Screening study
Language:
English
Year:
2015
Type:
Article
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