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Lys-413 of S-phase mRNA cycling sequence binding protein from Leishmania donovani (LdCSBP) is modified through monoubiquitination that is responsible for inhibition of its riboendonuclease activity.
Indian J Biochem Biophys ; 2014 Dec ; 51 (6): 559-566
Article in English | IMSEAR | ID: sea-156537
ABSTRACT
In addition to well-known process of proteasome-mediated degradation of polyubiquitinated proteins, monoubiquitination of proteins is also an important post-translational modification that regulates various non-degradative cellular processes like protein trafficking, cellular signalling, DNA replication and DNA repair. We have previously characterized a multi-domain cycling sequence binding protein LdCSBP from Leishmania donovani, which binds specifically to a conserved CAUAGAAG octamer containing RNAs via its uniquely arranged CCCH type Zn-fingers and degrades them using its Smr endonuclease domain, indicative of its potential role in the turnover of the S-phase mRNAs. Remarkably, its riboendonuclease activity is inhibited due to the incorporation of a monoubiquitin residue in the ZnF domain, though the target Lys residue remains unknown. Here, we report through systematic mutation of Lys residue to Ala that Lys-413 in LdCSBP is the site of monoubiquitination. However, the amino acid motif around the target Lys in LdCSBP is not consensus with any previously known monoubiquitination site, though partial homology is observed with a subset of recently identified mammalian ubiquitination target sites. Interestingly, Lys-413 of LdCSBP is conserved in the homologous annotated proteins from the related kinetoplastida parasites, suggesting similar monoubiquitination-mediated regulation of RNA endonuclease activity in the organisms.
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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Protein Binding / Structure-Activity Relationship / Binding Sites / Leishmania donovani / Molecular Sequence Data / Protozoan Proteins / S Phase / Zinc Fingers / Amino Acid Sequence / RNA-Binding Proteins Language: English Journal: Indian J Biochem Biophys Year: 2014 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Protein Binding / Structure-Activity Relationship / Binding Sites / Leishmania donovani / Molecular Sequence Data / Protozoan Proteins / S Phase / Zinc Fingers / Amino Acid Sequence / RNA-Binding Proteins Language: English Journal: Indian J Biochem Biophys Year: 2014 Type: Article