Auto antibodies against oxidized low density lipoproteins and lipid peroxidation in patients with essential hypertension

ABSTRACT

Lipid peroxidation has been suggested to play a key role in the oxidative modification of LDL (oxd-LDL) which stimulate the production of auto antibodies by Bcells and anti-oxd LDL antibodies are produced. These antibodies could represent a biological marker of oxidative stress and serve as markers of atherosclerosis. Essential hypertension is a major risk factor for atherosclerosis, recently border line hypertension also has been shown to be a risk factor for atherosclerosis. The aim of this study is to examine the correlation between oxd -LDL antibodies and lipid peroxidation in patient with essential hypertension. Blood samples were collected from patients with essential hypertension (n=155) and healthy individuals (n=160) levels of Malonaldihyde (MDA), Total cholesterol, Triglycerides, HDL and LDL were estimated by spectrophotometry and levels of Oxd- LDL antibodies were obtained by ELISA. Plasma levels of MDA, anti-oxdLDL antibodies, Total cholesterol and LDL Cholesterol is higher in patients than those in controls. Among patients concentration of MDA, total cholesterol and LDL cholesterol were not significantly different, however the concentration of anti-oxd LDL were higher in essential hypertensive patients (p=0.003). Significant positive correlation was observed between plasma levels of MDA, total cholesterol, LDL cholesterol and the concentration of anti-oxdLDL in patients but not in the controls. In conclusion High concentrations of anti-oxdLDL and MDA suggest an increase in oxidative stress that would contribute to the development of atherosclerosis. The observed correlation of MDA with anti-oxdLDL indicates the relationship between free radicals and atherosclerosis in essential hypertension.