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Comparative Study of Real-Time PCR with Culture in the Diagnosis of Tubercular Lymphadenopathy.
Article in English | IMSEAR | ID: sea-157698
ABSTRACT
One-third of the world’s population is estimated to be infected with M.tuberculosis. Timely and accurate diagnosis is pivotal in the management of the disease. Conventional tests, although accurate, are time consuming as compared to the more promising latest molecular techniques like real-time PCR which are rapid, reproducible and reliable.

Objective:

The aim of the study is to compare the real-time PCR with culture method in the diagnosis of tubercular lymphadenopathy. Material and

Methods:

Present study included 40 patients belonging to the age group 0-40 years and presenting as cervical lymphadenopathy, attending the indoor and out-patient of Department of TB & Chest Diseases, S.N. Medical College, Agra (U.P.) from Jan’2009 to June’2010. All patients were subjected to routine investigations, e.g. hemogram, montoux test, Chest skiagram, AFB staining, and sputum examination. Lymph node aspirate were subjected to decontamination, DNA isolation and Real time PCR( q PCR). Also specimen were simultaneously put to culture on L.J. Media. Results of both modalities compared.

Results:

Conventional culture method was able to detect M.tuberculosis in 75% of the cases as compared to real-time PCR which was positive in 77.5% with comparable sensitivity (100% vs 96.7%) and specificity (100% vs 87.5%).

Conclusion:

Conventional culture method is gold standard in the diagnosis of tuberculosis since long but recent molecular assays like Real-time PCR is one of the latest addenda to the armamentarium for the rapid and accurate diagnosis of M.tuberculosis. Needless to say, early diagnosis is advantageous to the management of tuberculosis.
Subject(s)

Full text: Available Index: IMSEAR (South-East Asia) Main subject: Tuberculosis / Female / Humans / Male / Infant, Newborn / Child / Child, Preschool / Adolescent / Adult / Early Diagnosis Type of study: Diagnostic study / Screening study Country/Region as subject: Asia Language: English Year: 2015 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Tuberculosis / Female / Humans / Male / Infant, Newborn / Child / Child, Preschool / Adolescent / Adult / Early Diagnosis Type of study: Diagnostic study / Screening study Country/Region as subject: Asia Language: English Year: 2015 Type: Article