Extraction and estimation of theobromine in marketed tea by HPTLC and UV method.

ABSTRACT

A rapid, simple and sensitive chromatographic method (RP-HPTLC) has been developed for the extraction and quantitative estimation of Theobromine in different extract tea samples (Camellia sinensis). Separation was performed on Silica gel 60 f254 HPTLC plates with ethyl acetate methanol (273 v/v), as mobile phase. The determination was carried out in the UV region, using densitometric absorbance at 274 nm. The maximum concentration of Theobromine in tea samples was found to be 2.313 %. The theobromine response was found to be linear over range 3-15 μg per zone. Limit of detection and quantitation were found to be 30 and 140 ng/ spot respectively. The HPTLC method was validated in terms of precision, accuracy, sensitivity and robustness. The proposed method provided precise and accurate analysis for extraction and estimation of theobromine by HPTLC-UV.