MALDI-TOF mass spectrometry proteomic based identification of clinical bacterial isolates.
Article
in English
| IMSEAR
| ID: sea-158376
ABSTRACT
Background & objectives:
Pathogenic bacteria often cause life threatening infections especially in immunocompromised individuals. Therefore, rapid and reliable species identification is essential for a successful treatment and disease management. We evaluated a rapid, proteomic based technique for identification of clinical bacterial isolates by protein profiling using matrix-assisted laser desorption-ionization time - of - flight mass spectrometry (MALDI-TOF MS).Methods:
Freshly grown bacterial isolates were selected from culture plates. Ethanol/formic acid extraction procedure was carried out, followed by charging of MALDI target plate with the extract and overlaying with α-cyano-4 hydroxy-cinnamic acid matrix solution. Identification was performed using the MALDI BioTyper 1.1, software for microbial identification (Bruker Daltonik GmbH, Bremen, Germany).Results:
A comparative analysis of 82 clinical bacterial isolates using MALDI -TOF MS and conventional techniques was carried out. Amongst the clinical isolates, the accuracy at the species level for clinical isolates was 98.78%. One out of 82 isolates was not in accordance with the conventional assays because MALDI-TOF MS established it as Streptococcus pneumoniae and conventional methods as Streptococcus viridans. Interpretation &conclusions:
MALDI - TOF MS was found to be an accurate, rapid, cost-effective and robust system for identification of clinical bacterial isolates. This innovative approach holds promise for earlier therapeutic intervention leading to better patient care.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Bacteria
/
Bacterial Infections
/
Humans
/
RNA, Ribosomal, 16S
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
/
Proteomics
Type of study:
Diagnostic study
Language:
English
Year:
2014
Type:
Article
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