Envelope specific T cell responses & cytokine profiles in chikungunya patients hospitalized with different clinical presentations.
Article
in English
| IMSEAR
| ID: sea-158451
ABSTRACT
Background & objectives:
Since the 2006 massive outbreaks, chikungunya (CHIK) is a major public health concern in India. The aim of this study was to assess envelope specific immune responses in patients with chikungunya infection.Methods:
This study included 46 hospitalized patients with chikungunya virus infection (encephalitis, n=22, other systemic involvement, OSI, n=12, classical, n=12) and six controls from Ahmedabad city, Gujarat, India. T cell responses and the levels of Th1, pro/ anti-inflammatory cytokines against the CHIK virus envelope antigens were assessed by lymphocyte proliferation assay and by cytometric bead array in flow cytometry, respectively.Results:
Lymphoproliferative response was uniform among the patients. Comparisons of cytokines revealed significantly higher levels of interleukin (IL)-4 and IL-5 in encephalitis, OSI and classical patients versus controls. The levels of tumour necrosis factor (TNF)-α were higher in classical patients categories compared to the controls. Interferon (IFN)-γ levels were lower in encephalitis patients versus control. Interpretation &conclusions:
Our findings showed recognition of T cell epitopes on the envelope region of chikungunya virus by all patient categories. Lower level of IFN-γ may be associated with the severity of disease in these patients.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Humans
/
T-Lymphocytes
/
Cytokines
/
T-Lymphocyte Subsets
/
Chikungunya Fever
/
India
Country/Region as subject:
Asia
Language:
English
Year:
2015
Type:
Article
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