Production of 4-Ipomeanol, an anticancer agent from the root tubers and rhizogenic callus of Ipomoea batatas Lam.: A Comparative study.
Indian J Exp Biol
;
2015 May; 53(5): 297-304
Article
in English
| IMSEAR
| ID: sea-158455
ABSTRACT
A comparative study was done on the production of 4-ipomeanol from root tubers of Ipomoea batatas and its rhizogenic callus. Best callusing response was obtained on MS medium supplemented with 11 µM NAA (α-Naphthaleneacetic acid) and 1 µM KIN (Kinetin). Effect of various elicitors (Fusarium solani, chitin and chitosan) on the production of 4-ipomeanol was studied. Methanol extract of the samples were purified by column chromatography and detected using TLC. Identification of 4-ipomeanol was confirmed using HPLC and quantified spectrophotometrically. A mass spectrum was recorded to confirm the presence of 4-ipomeanol. The calli grown under chitin produced highest (6.61mg g-1) amount of 4-ipomeanol. This is the first report on in vitro production of 4-ipomeanol from I. batatas. Since 4-ipomeanol is reported to be present only in I. batatas, this study would help in standardizing protocols for large scale production without affecting its natural flora.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Terpenes
/
Chitin
/
Plant Roots
/
Ipomoea batatas
/
Plant Tubers
/
Chitosan
/
Culture Techniques
/
Antineoplastic Agents
Language:
English
Journal:
Indian J Exp Biol
Year:
2015
Type:
Article
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