In vitro study on the efficacy of zinc oxide and titanium dioxide nanoparticles against metallo beta-lactamase and biofilm producing Pseudomonas aeruginosa.

ABSTRACT

Pseudomonas aeruginosa contributes to a multitude of infections exhibiting intrinsic resistance to numerous antibiotics. Metallo beta-lactamase and biofilm production is the most worrisome resistant mechanisms observed in P. aeruginosa. Emergence of antimicrobial resistance by pathogenic bacteria is a major health problem in recent years. Zinc oxide and titanium dioxide nanoparticles comprises of well-known inhibitory and bactericidal effects. The present study is designed to determine the efficacy of zinc oxide and titanium dioxide nanoparticles against metallo beta-lactamase and biofilm producing P. aeruginosa. A total of 51 non-repetitive P. aeruginosa isolates were obtained from Bioline laboratory, Coimbatore. Biofilm and metallo beta-lactamase production was tested by combined disc test and tissue culture plate method. Commercially available zinc and titanium dioxide nanoparticles were obtained and tested against two metallo beta-lactamase and biofilm producing isolates. Both the nanoparticles showed appreciable activity at all tested concentrations. Thus, it is concluded that ZnO and TiO2 nanoparticles may serve as a promising antibacterial agents in coming years.