In Vitro α-Amylase Inhibition and Antioxidant potential of Chloroform Fraction of Hydroalcoholic Extract Obtained from Hyptis Suaveolens.

ABSTRACT

The plant Hyptis is an effective medicinal herb and a well known medicinal plant in herbal world. The present study was aimed to investigate the α-amylase inhibition and antioxidant activities of chloroform fraction of H. suaveolens. Chloroform fraction of H. suaveolens was screened for α-amylase inhibition activity by 3, 5- dinitrosalicylic acid (DNSA) method respectively. and antioxidant activity was evaluated by 1,1-diphenyl-2- picryl-hydrazile (DPPH) free radical scavenging, Super oxide radical Scavenging, nitric oxide (NO) radical scavenging, and 2.2’-azinobis-3-ethylbenzothiazole-6-sulfonic acid (ABTS) radical scavenging assays. The chloroform fraction of H. suaveolens showed effective α-amylase inhibition activity (IC50 57.34μg/ml). Chloroform fraction demonstrated significant antioxidant activity in all the in vitro antioxidant models. Moreover, the chloroform fraction was found to be extremely effective in scavenging DPPH (IC50 57.51 μg/ml) while compared to super oxide (IC50 61.36 μg/ml), NO (IC5076.3 μg/ml) and ABTS radical (IC50 93.16 μg/ml) scavenging activity. In conclusion, from the results of present study it is established that antioxidant and alphaamylase inhibitory activity of chloroform fraction of H. suaveolens may contribute in its earlier observed antidiabetic potential.