Phylogenetical metabolite logical algorithm: A test using phylogenetic metabolite analysis using TLC in some convolvulaceae members.

ABSTRACT

Chemical substances act as vital substances occurring in living organisms and can be studied through various biotechnological approaches. The characteristic metabolites of a plant species may reflect evolution towards the resistance produced to environment. Evolvulus alsinoides, Evolvulus nummularius and Merrimia tridentata belongs to convolvulaceae members has been taken as experimental plants in the present study. Thin layer chromatography (TLC) has been used as a data collection technique for identification of similar organisms by logical method, from various Rf values occurred by experimentation. Based on phylogenetic analysis using UPGMA method, E.nummularius has shown good matches with both E.alsinoides and M.tridentata. Hence E.nummularius may be showing evolutionary link between E.alsinoides and M.tridentata. Seventeen compounds are visualized with similar Rf values between all these plant members. Hence most of the data are observed to be having similar compounds, which makes to be placed in same family (convolvulaceae).