In-vitro Assessment of Effectiveness and Photostability Avobenzone in Cream Formulations by Combination Ethyl Ascorbic acid and alpha Tocopherol Acetate.

ABSTRACT

Avobenzone is UVA sunscreen active substances, which are unstable when exposed to UV radiation, especially UVA. Theaim of this study is to determine the effectiveness and photo stability avobenzone when combined with ethyl ascorbic acid and alpha tocopherol acetate with various concentrations of ethyl ascorbic acid and alpha tocopherol by in vitro using spectrophotometric method. The photostability study of the eight variations of formula showed that the formula3, 4, 5, 6, 7 and 8 had significant differences with Formula 1 (p<0,05) and the best photostability represented by the formula 4 (avobenzone 2%, ethyl ascorbic acid2%) with avobenzone concentrations decrease by 11,82% for 15 hour using UVA lamp irradiation 4,7 mW/cm2. In effectiveness of SPF (Sun Protection Factor) value determination showed moderate protection category (SPF ±6). The percent transmission of erythema and pigmentation test showed that cream had effectiveness as sunscreen by showing the category as sun block on the area pigmentation and not to erythema areas at concentration 1000 μg/ml.