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Time And Temperature Effects On In-Vitro Sperm Chromatin and Stability.
Indian J Hum Genet ; 1999 Jan; 5(1): 51-55
Article in English | IMSEAR | ID: sea-159881
ABSTRACT
Semen samples were collected both from men with or without children. Sperm wash preparations were made and the samples were studied at different temperatures and times of incubations for genome packaging efficiency. It is found that sperm chromatin decondensation study could be optimised with SDS reagent if the temperature was 37 degrees and time of incubation was 30 minutes. Similarly, SDS & EDTA, optimum time was 15 minutes at body temperature, thus affecting results of the study. Below, the body temperature the reaction is slow and does not mimick the in-vivo process.

Full text: Available Index: IMSEAR (South-East Asia) Language: English Journal: Indian J Hum Genet Year: 1999 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Language: English Journal: Indian J Hum Genet Year: 1999 Type: Article