Effect of phospholipase-D on rat kidney mitochondria.
J Biosci
;
1979 Mar; 1(1): 75-82
Article
in English
| IMSEAR
| ID: sea-159932
ABSTRACT
Incubation of purified rat kidney mitochondrial fraction with phospholipase- D resulted in the accumulation of phosphatidic acid in the membrane due to the degradation of membrane-bound phosphatidylcholine, -serine and -ethanolamine Simultaneously with the hydrolysis of the phospholipids, cholesterol and protein were released from the mitochondrial membrane into the medium, and binding of Ca2+ by mitochondrial membranes increased. Phospholipase Dtreated mitochondrial fraction exhibited increased swelling in vitro in the early stages of incubation (15 min) after which the mitochondria were ruptured. Membrane- bound adenosine triphosphatase was partially inactivated and the enzyme activity was not significantly restored by incubation with sonicated dispersions of phosphatidylcholine, -serine and cholesterol. These results indicate that removal of choline, serine and ethanolamine from membrane-bound phospholipids disrupt phospholipid-cholesterol and phospholipid-protein association and affect functions of the membrane.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Language:
English
Journal:
J Biosci
Year:
1979
Type:
Article
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