Isolation and characterisation of cathepsin-B from bovine pancreas.
J Biosci
;
1979 Dec; 1(4): 401-408
Article
in English
| IMSEAR
| ID: sea-160041
ABSTRACT
Α simple procedure for the isolation of cathepsin B from bovine pan creas employing ammonium sulphate fractionation, DEAE cellulose chromatography and Sephadex G 200 gel filtration is described. The purified enzyme gave a single band on polyacrylamide gel electrophoresis. The molecular weight as determined by gel filtration of the enzyme was 26,850. Its Km and Vmax values were 12·8 mM and 0·303 μmol/min/mg, respectively. The Ki for iodoacetamide was 0·16 mM.
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Index:
IMSEAR (South-East Asia)
Language:
English
Journal:
J Biosci
Year:
1979
Type:
Article
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