Regulation of phosphoglycerate mutase in developing forespores and dormant spores of Bacillus megaterium by the in vivo levels of phosphoglycerate mutase inhibitor.
J Biosci
;
1982 Dec; 4(4): 431-439
Article
in English
| IMSEAR
| ID: sea-160180
ABSTRACT
Bacillus megaterium accumulated 3-phosphoglycerate during sporulation which was utilized during spore germination. During sporulation a protein was synthesized before or at the start of 3-phosphoglycerate accumulation inside the developing spores about 1.5 h before dipicolinic acid accumulation. This protein has an affinity for Mn2+ and other divalent metal ions and inhibits phosphoglycerate mutase activity which has been shown to require Mn2+ However, the levels of the inhibitor decreased considerably (75-85%) during spore germination. No appreciable amount of the inhibitor was detected in the vegetable cell and mother cell compartment; however, the forespore compartment possesses an activity comparable to that of dormant spores. The partially purified inhibitor has a molecular weight of 11,000 and possesses both high and low affinity binding sites for Mn2+ and Ca2+ as determined by Scatchard plot analysis.
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Index:
IMSEAR (South-East Asia)
Language:
English
Journal:
J Biosci
Year:
1982
Type:
Article
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