Monoclonal antibodies against Wuchereria bancrofti microfilarial excretory-secretory antigens.

ABSTRACT

A battery of monoclonal antibodies were produced against Wuchereria bancrofti microfilarial excretory-secretory antigens and their specificity was studied using different filarial antigens. Among the 1116 wells plated out, 42 % of the wells developed hybrids and 5 % of the hybrids showed anti Wuchereria bancrofti microfilarial excretory-secretory antigens. Specificity studies on the antibodies produced from 63 cloned and expanded hybrids showed 10 clones which were specifically positive only to Wuchereria bancrofti microfilarial excretory-secretory antigens.