Influence of formamide on the thermal stability of DNA.
J Biosci
;
1984 Dec; 6(6): 817-821
Article
in English
| IMSEAR
| ID: sea-160425
ABSTRACT
The utility of formamide in the denaturation and renaturation of DNA has been examined. The melting temperature of duplex DNA is lowered by 0·6oC per per cent formamide. The depression of melting temperature is independent of the GC content. Formamide also increases the width of the thermal transition. Upto 30%, it does not affect the rate of DNA reassociation.
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Index:
IMSEAR (South-East Asia)
Language:
English
Journal:
J Biosci
Year:
1984
Type:
Article
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