Isolation and characterization of cDNA clones for α- and β-subunits of ovine luteinizing hormone.
J Biosci
;
1987 Dec; 12(4): 349-357
Article
in English
| IMSEAR
| ID: sea-160610
ABSTRACT
A cDNA library of ovine pituitary DNA in plasmid pBR322 has been constructed by conventional methods with certain modifications. The library was screened using partial cDNAs for rat α-subunit and LHβ. We have isolated cDNA clones for ovine α- subunit and LHβ. The identification of these clones was confirmed by partial sequencing. The clones bear about 80% sequence homology with the respective rat cDNAs in the sequenced regions and hybridize with the rat clones in 5 X SSC at 55°C. The ovine LHβ clone has an insert of about 650 bp and selects an RNA of about 750 bases in a northern blot. The α-subunit cDNA clone has an insert of about 550 bp; it has two internal Pst I sites and thus shows restriction-based differences from rat α-subunit cDNA, which does not have any Pst I site.
Full text:
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Index:
IMSEAR (South-East Asia)
Language:
English
Journal:
J Biosci
Year:
1987
Type:
Article
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