Stick enzyme-linked immunosorbent assay using the avidin–biotin system for detection of circulating antigen in bancroftian filariasis.
J Biosci
;
1988 Sep; 13(3):229-233
Article
in English
| IMSEAR
| ID: sea-160671
ABSTRACT
Detection of filarial antigen in different groups of sera was carried out by sandwich as well as inhibition enzyme-linked immunosorbent assays using antibody-coated sticks. Both systems were found to be equally sensitive in detecting antigen in 90% of microfilariae carriers. Incorporation of avidin-biotin in the sandwich assay system increased the sensitivity of antigen detection from 10–6 to 10–16 pg. A 67% decrease in the number of false negative results was observed when the sensitive avidin-biotin inhibition enzymelinked immunosorbent assay system was used for analysis of filaria blood samples.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Type of study:
Diagnostic study
Language:
English
Journal:
J Biosci
Year:
1988
Type:
Article
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