Keratinization of rat vaginal epithelium IV. Modulation of transglutaminase activity by oestradiol.
J Biosci
;
1988 Dec; 13(4): 401-408
Article
in English
| IMSEAR
| ID: sea-160697
ABSTRACT
Calcium-dependent transglutaminase activity was found to be present in vaginal homogenates from adult cycling rats. Treatment of immature or adult ovariectomized rats with oestradiol (0·1 μg/g body weight) resulted in 1·5–2-fold enhancement in the enzyme activity. Progesterone treatment (0·1 μg/g body weight) decreased the enzyme activity. Analysis of amino acids produced by proteolytic enzyme digestion of insoluble keratins from rat vaginal tissue indicated the presence of γ-glutamyl-ε-\lysine dipeptide (4 μmol/g protein) in this protein. These results suggest that oestradiol acts on vaginal tissue and induces the activity of transglutaminase. This enzyme catalyses the formation of γ-glutamyl-ε-lysine crosslinks between keratin polypeptides and thus leads to kerartization of the tissue.
Full text:
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Index:
IMSEAR (South-East Asia)
Language:
English
Journal:
J Biosci
Year:
1988
Type:
Article
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