Changes in cytoplasmic pH upon heat shock in embryonic and adult rat liver ceils.

ABSTRACT

All living cells, when exposed to elevated temperatures, undergo physiological changes which result in the expression of a specific set of heat shock proteins. Study of the possible physiological changes in adult and embryonic rat liver cells indicated a change in intracellular pH upon heat shock. Using 2', 7'-bis (2-carboxyethyl)-5 (and -6) carboxyfluorescein acetoxymethyl ester, we demonstrate here that the intracellular pH of adult and embryonic liver cells is different and that there is an increase in relative fluorescence intensity in both adult and embryonic cells upon heat shock, which corresponds to about 0·2 to 0·3 pH units. We also show that in addition to heat, some of the inducers of heat shock like response in many systems also induce a change in intracellular pH and induce heat shock proteins at 37°C in fetal liver cells. The possible mechanisms of induction of heat shock proteins during heat shock and in the presence of inducers at normal temperature are discussed.