Structural organization of the transfer RNA gene clusters of cholera phage φ 149.

ABSTRACT

Vibrio cholerae phage φ l49 codes tRNAs specific for twelve different amino acids. These tRNA genes are contained in two different HindIII fragments 11 and 3·4 kb in size, of the phage genome. The 3·4 kb HindIII fragment was cloned in Escherichia coli using pBR328 as vector. The recombinant plasmid pNR347 produced nine of the twelve tRNA species (arginine, proline, serine, tyrosine, histidine, lysine, leucine, tryptophan and aspartic acid) encoded in the phage genome.