Mutagenesis study to ameliorate the bacterial expression of phosphoprotein P of vesicular stomatitis virus.
J Biosci
;
1996 Sept; 21(5): 687-697
Article
in English
| IMSEAR
| ID: sea-161137
ABSTRACT
The phosphoprotein gene of vesicular stomatitis virus, a Rhabdovirus, has been inserted into bacterial expression plasmids containing the Escherichia coli tac promoter and ribosome binding site (RBS). A low level of expression of the protein was detected. Sequence analysis showed the presence of 15 nucleotides in the spacer region i.e., between the Shine- Dalgarno sequence and ATG. Alteration of the distance and the sequence in the spacer region by oligonucleotide-directed mutagenesis revealed a correlation among the expression levels, accessibility of the RBS and requirement for a minimum spacing of at least 7 nucleotides between the Shine-Dalgarno sequence and ATG for optimal gene expression.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Language:
English
Journal:
J Biosci
Year:
1996
Type:
Article
Similar
MEDLINE
...
LILACS
LIS