A facile method for the construction of synthetic genes.
J Biosci
;
1996 Dec; 21(6): 735-741
Article
in English
| IMSEAR
| ID: sea-161146
ABSTRACT
A simple protocol for rapid assembly of chemically synthesized deoxyoligonucleotides into double stranded DNA is described. Several parameters of a ligation-free method were investigated to allow efficient assembly of a large number of oligonucleotides into double stranded DNA by polymerase chain reaction. Synthesis of a 701 bp DNA was carried out in a single reaction by assembling 28 oligonucleotides designed with partial overlaps at complementary ends. An estimate of error rate was made by sequencing several independent clones of the synthesized DNA.
Full text:
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Index:
IMSEAR (South-East Asia)
Language:
English
Journal:
J Biosci
Year:
1996
Type:
Article
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