In vitro shoot development in blepharis repnes.

ABSTRACT

An herb is a plant or plant part valued for its medicinal aromatic or savory qualities. In vitro shoot development of Blepharis repnes was achieved using thin sections of nodes and stems (1.0-1.5 mm) were cultured in MS medium containing varying concentrations of different combination of phytohormones Indole-3-butyric acid (IBA), Benzylaminopurine (BAP) & Gibberlic acid. The combination of BAP, IBA & Gibberellic acid at different concentrations showed the length of shoot development 0.6 -1.8cm with in three weeks in suitable laboratory condition.