Mapping of additional restriction enzyme cleavage sites on bacteriophage MB78 enzyme.

ABSTRACT

A physical map of bacteriophage MB78 DNA indicating the cleavage sites for the enzyme Bg/II, ClaI, EcoRI, Pvull, Sa/! and SmaI comprising of a total of 34 cleavage sites have been constructed earlier. The cleavage sites for a few more restriction endonucleasesli ke ApaI, AvaI, Bg/!, Hindlll, KpnI and XhoI have now been mapped. A total of 72 cleavage sites on MB78 DNA are known by now. Relative positions of EcoRI I and J fragments which could not be decided earlier has now been determined.