Recombinant expression of human transforming growth factor-ß isoforms in Chinese hamster ovary cells. combinant expression of human transforming growth factor-ß isoforms in Chinese hamster ovary cell.

ABSTRACT

Transforming growth factor-ps (TGF-ps) are multi functional growth modulators implicated in several physiological processes which include embryogenesis, inflammation, immune-suppression, wound healing, carcinogenesis and cellular differentiation. For clinical use, recombinant expression of TGF-ps is the only practical source because of very low yields from natural sources. Here, we report the recombinant expression of human TGF-pl and TGF-p2 in a mammalian expression system using a high expression eukaryotic vector driven by a cytomegalovirus promoter. Expression levels are as high as 0.97 flg/ml of TGF-pl and 0.24 flg/ml of TGF-p2 in conditioned media, sufficient for purification without the need for amplification of the gene using methotrexate.