Crowding, molecular volume and plasticity: An assessment involving crystallography, NMR and simulations.
J Biosci
;
2012 Dec; 37 (6): 953-963
Article
in English
| IMSEAR
| ID: sea-161763
ABSTRACT
The discrepancy between the X-ray and NMR structures of Mycobacterium tuberculosis peptidyl-tRNA hydrolase in relation to the functionally important plasticity of the molecule led to molecular dynamics simulations. The X-ray and the NMR studies along with the simulations indicated an inverse correlation between crowding and molecular volume. A detailed comparison of proteins for which X-ray and the NMR structures appears to confirm this correlation. In consonance with the reported results of the investigations in cellular compartments and aqueous solution, the comparison indicates that the crowding results in compaction of the molecule as well as change in its shape, which could specifically involve regions of the molecule important in function. Crowding could thus influence the action of proteins through modulation of the functionally important plasticity of the molecule.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Language:
English
Journal:
J Biosci
Year:
2012
Type:
Article
Similar
MEDLINE
...
LILACS
LIS