Ruthenium (II) complexes: Physico-chemical, spectroscopic, redox, microbial and DNA binding studies.

ABSTRACT

An octahedral ruthenium(II) Schiff base complexes of the type [Ru(CO)(Py)L] (L = dianion of the Schiff bases derived from acetoacetanilide with o-phenylenediamine and salicylaldehyde/ohydroxyacetophenone/ o-vanillin/2-hydroxy-1-naphthaldehyde) have been synthesized from the reactions of equimolar ratio of [Ru(CO)(PPh3)2(Py)] and Schiff bases in benzene. The formation of the Schiff base ligands and its complexes have been envisaged from IR, UV-VIS, 1H, 13C, 31P NMR, High resolution mass and Powder XRD studies. These spectral studies confirm an octahedral environment around the metal ion. The redox behaviour of the complexes has also been determined. The ligands, metal precursors and the complexes were tested for their efficiency towards antimicrobial activity. DNA binding studies (Herring Sperm DNA) were carried out for the complexes [Ru(CO)(Py)L1] and [Ru(CO)(Py)L2] using biochemical techniques such as UV-VIS, cyclic voltammetry and differential pulse voltammetry. These techniques paved the way to probe the details of their DNA binding abilities. Intrinsic binding constant have been estimated and it showed a moderate intercalative interactions than the other classical intercalators.