Rapid micropropagation of Cucumis sativus var. Dastgerdi (Iranian cultivar) by Node Culture Technique.
Br Biotechnol J
;
2014 Jun; 4(6): 733-739
Article
in English
| IMSEAR
| ID: sea-162472
ABSTRACT
Tissue culture can be used in developing an efficient method for production of valuable and enduring indigenous cultivars. A short-term protocol for plantlet proliferation by using nodal segments was developed for Cucumis sativus, local cultivar from Isfahan; known as Dastgerdi as a tolerant cultivar to the root-knot nematode. Nodal explants were cultured on MS medium containing various concentrations of KIN (0, 0.5, 1 and 1.5mg.l-1) in combination with IBA (0, 0.025 and 0.5mg.l-1). The best response for proliferation rate (100%), mean number of nodes per plantlet (6.01) and percentage of rooted In vitro propagated shoots were observed on medium supplemented by KIN (1.5mg.l-1) after about 3-4 weeks. The maximum length (5.43cm) of micro-shoots obtained on MS medium with KIN (1mg.l-l) + IBA (0.025mg.l-1).
Full text:
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Index:
IMSEAR (South-East Asia)
Language:
English
Journal:
Br Biotechnol J
Year:
2014
Type:
Article
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