Efficient Micropropagation of Spilanthes acmella (L.) Murr.: A Threatened Medicinal Herb.
Br Biotechnol J
;
2013 July; 3(3): 405-415
Article
in English
| IMSEAR
| ID: sea-162515
ABSTRACT
The present study describes an efficient and reproducible protocol for micropropagation of S. acmella. Shoot tips taken from 3 week-old aseptic seedlings were cultured on Murashige and Skoog (MS) semi-solid medium supplemented with different concentrations of TDZ. Among various concentrations, 0.25 μM TDZ was found to be optimum for shoot regeneration as it induced a maximum of 30.0 shoots per explant however with retarded growth (1.0 cm). Among different volumes of culture media, 15 ml liquid culture medium favored best response wherein a maximum of 80.2 shoots per explant with an average shoot length of 7.0 cm were induced after 6 week of subculturing. Successful in vitro rooting was induced on 2.5 μM NAA containing half-strength MS medium. Almost 96% rooted plants successfully transferred and acclimatized ex vitro under green house conditions. Morphological and physiological parameters compared with the in vivo-grown seedlings of the same age appeared to be ‘normal’ in respect to the fundamental characteristics examined.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Language:
English
Journal:
Br Biotechnol J
Year:
2013
Type:
Article
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