Genetic Characterization of Insulin Growth Factor-1 and Its Receptor Genes in Egyptian Buffalo (Bubalus bubalis L.).
Br Biotechnol J
;
2013 Oct; 3(4): 592-604
Article
in English
| IMSEAR
| ID: sea-162547
ABSTRACT
Aim:
The somatotropic axis (SA) comprises genes associated with economically important quantitative traits in livestock like mammary and muscle growth as well as carcass traits. Insulin growth factor-1 (IGF-1) and its receptor (IGF-1R) are two important genes belonging to the SA. The aim of this study was to evaluate the genetic polymorphism of IGF1/SnaBI and IGF-1R/TaqI restriction sites in Egyptian buffalo.Methodology:
Genomic DNA was extracted from blood samples of 100 healthy buffaloes maintained at the Mahlet Mussa and El-Gmeasa herds from 2010 to 2012. PCR was performed using primers flanking a 250-bp fragment of the regulatory region of the buffalo IGF-1 gene and a 616-bp fragment of the IGF-1R gene encompassing 51-bp from exon 12, 479-bp from intron 12 and 86-bp from exon 13. The PCR-amplified fragments were digested with SnaBI (IGF-1) and TaqI (IGF-1R), electrophoresed and analyzed on agarose gels stained with ethidium bromide. The two amplified fragments were also sequenced and aligned with published sequences.Results:
All buffaloes investigated in this study were genotyped BB (i.e., negative for the SnaBI restriction site at position 224
Full text:
Available
Index:
IMSEAR (South-East Asia)
Language:
English
Journal:
Br Biotechnol J
Year:
2013
Type:
Article
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