Detection of FUS-1 (OXA-85), a Class D Betalactamase from Fusobacterium nucleatum Subspecies Polymorphum in Nigeria.
Article
in English
| IMSEAR
| ID: sea-162936
ABSTRACT
Aims:
Beta-lactamase production and subsequent resistance to β-lactam drugs has been a global concern in the treatment of Gram negative anaerobes. The aim of this study was to identify F. nucleatum strains producing Class D β-lactamase through the detection of FUS-1 (OXA-85) resistance gene. Place and Duration of Study Department of Preventive Dentistry, Lagos University Teaching Hospital, Idi-Araba, between February 2010 and November 2010.Methodology:
Twenty two oral clinical samples were obtained from patients with chronic periodontitis who admitted to previous use of amoxicillin. Antibacterial susceptibility of the bacterial isolates was determined by E-test on Brucella Blood agar. Amplification of the bacterial DNA was carried out by PCR using F. nucleatum species-specific primer, FUS-1 specific for blaFUS-1 and strain-specific primers for subspecies nucleatum,, fusiforme, polymorphum and vincentii.Results:
From the 19 samples collected, F. nucleatum was isolated, and the identity of the isolates was confirmed by PCR. Four of the isolates produced similar bands with the control strain, 3 (15.7%) strains were able to produce amplication with FUS-1 primer specific for blaFUS-1 gene found in β-lactamase producing F. nucleatum subsp. polymorphum.Conclusion:
This study shows the presence of class D β-lactamase producing F. nucleatum species in Nigeria.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Bacteria
/
Beta-Lactamases
/
Female
/
Humans
/
Male
/
Child
/
Fusobacterium nucleatum
/
Adult
/
Genes, Bacterial
/
Middle Aged
Type of study:
Diagnostic study
Country/Region as subject:
Africa
Language:
English
Year:
2013
Type:
Article
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