Evidences for Deleterous Role of Free Radicals in Experimental Varicocele Using Animal Model.
Br J Med Med Res
;
2013 Oct-Dec; 3(4): 1125-1143
Article
in English
| IMSEAR
| ID: sea-162981
ABSTRACT
Aim:
It is highly intricate to categorize a solitary or prevailing factor for pathophysiology of varicocele. Herein, the basis of free radicals in the pathogenesis of varicocele was assessed. StudyDesign:
Experimental using animal models. Place and Duration of Study Department of Anatomy, College of Medicine, Lagos State University, Ikeja, Lagos, Nigeria, between April, 2012 and August, 2012.Methodology:
Five (5) groups of rats were used, Group A animals served as the control, while Groups B, C, D and E animals were varicocelized. Groups C, E and E in addition, had intramuscular treatment of 25 mg/kg, 50 mg/kg and 75mg/kg body weight of α-tocopherol respectively. The models were sacrificed on 65th day and Testicular weights and volumes, sperm parameters, histology, morphometry, enzymatic and non enzymatic antioxidants were vastly estimated.Result:
There was a significant (p<0.05) increase in activity level of SOD (5.92±4.1), CAT (380.2±7.1) and GPx (0.79±0.8) and a reduced lipid peroxidation evidenced by significant (p<0.05) reduction in level of MDA (18.2±6.1) of the varicocelized rat treated with Vitamin E (75mg/kg b.wt.) when compared to the activity of SOD (3.31±4.1), CAT (361.2±4.5), GPx (0.36±6.1) and MDA (0.36±6.1) of untreated varicocelized models. The geometric values, sperm characteristics and histological profiles threaded the same pattern as the oxidative status.Conclusion:
These results confirmed and validated the important role of reactive oxygen in the pathogenesis of varicocelized.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Type of study:
Prognostic study
Language:
English
Journal:
Br J Med Med Res
Year:
2013
Type:
Article
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