First Isolation and Identification of Listeria monocytogenes Isolated from Frozen and Shock Frozen Dressed Broiler Chicken in Sudan.

ABSTRACT

Aim: To isolate and identify Listeria monocytogenes from frozen and shock frozen raw dressed broiler chicken in Khartoum State, Sudan. Place and Duration of Study Department of Pathology, Microbiology and Parasitology, Sudan University of Science and Technology, Khartoum-North, Sudan, between July 2011 and June 2012. Methodology: Eight hundred samples were used in this the study. Five hundred frozen (-18º) raw dressed broiler chickens samples were collected from five station chicken abattoirs in Khartoum State. Three hundred samples were collected as fresh, -18ºC and shock frozen (-40ºC) raw dressed broiler chickens. Detection and isolation of L. monocytogenes was carried out using the conventional International Organization for Standardization method. Results: Out of the 500 samples, 195(39%) were found to be contaminated with Iisteria spp.; L. monocytogenes 64(12.8%), Listeria ivanovii 97(19.4%), Listeria grayi 20(4%), Listeria seeligeri 5(1%), Listeria welshimeri 9(1.8%). Out of the 300 samples, 111(37%) were found to be contaminated with Iisteria spp.; L. monocytogenes 39(13%), Listeria ivanovii 54(18%), Listeria grayi 11(3.6%), Listeria seeligeri 3(1%) and Listeria welshimeri 4(1.3%). Conclusion: The results presented in this study indicated that L. monocytogenes was found in frozen (-18ºC) raw dressed broiler chicken and shock frozen (-40ºC) raw dressed broiler chickens.