Simultaneous Determination of Hyoscine N- Butyl Bromide and Paracetamol by RP-TLC Spectrodensitometric Method.
Article
in English
| IMSEAR
| ID: sea-163233
ABSTRACT
Aims:
A simple RP-TLC Spectrodensitometric method was developed for determination of Hyoscine N-Butyl Bromide (HBB) and Paracetamol (PAR) either in bulk powder or in their pharmaceutical preparation. StudyDesign:
Validation study.Methodology:
In this method, HBB and PAR were separated on RP-18 W/ UV254 TLC plates using developing mobile phase consisting of methanol citrate buffer (pH=1.5) triflouroacetic acid (70300.1, by volume) at room temperature. Experimental conditions such as band size, slit width, different developing systems and scanning wavelength were carefully studied and the optimum conditions were selected. The obtained bands were then scanned at 210 nm. The two drugs were satisfactorily resolved with RF 0.60 ± 0.02 for HBB and 0.81 ± 0.02 for PAR. The validation of spectrodensitometric method was done regarding linearity, accuracy, precision, and specificity.Results:
Linearity of the proposed methods was evaluated and it was found to lie within the concentration range of 2.0-12.0 μg.band-1 for HBB and 2.0-14.0 μg.band-1 for PAR.Conclusion:
The proposed method was successfully applied for determination of HBB and PAR in pure form and in their different pharmaceutical formulations. The method proved to be specific, accurate and selective.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Spectrophotometry
/
Butylscopolammonium Bromide
/
Chemistry, Pharmaceutical
/
Chromatography, Thin Layer
/
Analgesics, Non-Narcotic
/
Acetaminophen
Language:
English
Year:
2013
Type:
Article
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