Isolation and charecterization of an antimicrobial protein from the liver of goat (capra hircus)

ABSTRACT

Antimicrobial proteins (AMPs) have become recognized as important components of the non specific host defense or innate system in a variety of organisms including bacteria, fungi, plants, insects, birds, amphibians, mammals and crustaceans. In the present study an AMP was isolated from the liver of CAPRA HIRCUS (Goat). Protein was isolated from the freshly collected liver tissue. Further acidic, basic and neutral fractions of protein were obtained and their antimicrobial activity was checked against B. Subtilis, E. Coli, P. Vulgaris and Enterobacter. The protein fractions having antimicrobial activity was subjected to SDS PAGE. The separated fractions were further processed for mass spectroscopy (LCQ-Fleet Mass Spectrophotometer) and their mass was deduced by the spectral data analysis. The amino acid sequence (FGGGMA) was deduced from the peaks obtained in mass spectroscopy which was having mass of 0.8 KD.