Multidrug resistant gram negative pathogens antibiotic profile and its effective control using secondary metabolites from marine actinobacteria.

ABSTRACT

Aim: To screen the spread of resistance in ESBLs producer’s particularly non lactose fermenting gram negative Acinetobacter spp. and Pseudomonas spp. and study antimicrobial activity with crude extract from novel marine actinomycetes in India. Methods: Fifty clinical isolates in a period of one year were processed and the antibiotic susceptibility was determined by double disk approximation test, the ESBLs production was screened with phenotypic confirmatory methods using disks of amikacin, meropenem, netilimicin, ciprofloxacin, gentamicin, tigecycline and piperacillin along with cephalosporin disks. Antimicrobial activity of the crude extract was determined by agar plug method. Results: The isolates collected from different samples were found resistant to third and fourth generation cephalosporins. ESBL production was detected in 56 % to 66 % of the isolates, amikacin and netilmicin showed 50% to 60% resistance they were also found resistant to carbapenems, 86% resistance was observed in Acinetobacter spp. Two strains PM21 and PM27 selected from 24 actinobacterial isolates had zone of inhibition >21mm. Conclusion: A high level of antibiotic resistance was found in Acinetobacter spp. in our study and may reflect the scenario in India. Earlier detection and reporting of ESBL producers will help in treating individual cases and also in controlling the spread of these resistant genes to other sensitive nosocomial isolates. The medical need for new agents is most acute and the future of this work aims to identify one such novel compound from marine actinobacteria.