Studies on the antioxidant activity of actinorhytis calapparia.

ABSTRACT

In the present study, preliminary phytochemical screening and in-vitro antioxidant activity of aqueous and methanolic fruit extracts of Actinorhytis calapparia H.Wendl. & Drude was investigated. The antioxidant activity was studied by using in vitro antioxidant models viz., DPPH radical scavenging activity and reducing power assay. Both the extracts showed antioxidant activity by inhibiting DPPH free radicals and also showed reducing power ability in ferric reducing model which was a dose dependent. The IC50 value was found to be 15 and 26 μg/ml for methanolic and aqueous extract respectively. Phytochemical screening of the extract revealed the presence of tannins, steroids, carbohydrates and amino acids. The total phenolic content of the extract was determined using the Folin-Ciocalteu method. The total phenolic content observed for aqueous and methanolic extracts were 56 and 64.3 mg/g equivalent of gallic acid respectively.