Optimization of cultural conditions for exopolysaccharides production by Frateuria aurentia.

ABSTRACT

The exopolysaccharides (EPS) producing ability was evaluated by nutritional defined media by the Frateuria aurentia was the highest yield EPS-producing strain, which was isolated from elephant dung in Srivilliputhur, Tamilnadu, India. It was identified as Frateuria aurentia with carbohydrate assimilation process and 16S rDNA gene sequencing. The favourable conditions for EPS yield by the microorganism growth in a chemically defined medium were tested with carbon, nitrogen, cultivation time, temperature, pH, hydrocarbon, surfactants, sodium chloride, metal ions and inoculums concentration in static and shaking conditions had the most significant influences. Among the various nutritional sources of interactive effects the maximum EPS production was recorded at pH 7.00 (1.431 ± 0.0096 OD); 30 C (1.661 ± 0.028 OD); Jaggery (1.185 ± 0.003 OD); ⁰ Tryptone (1.248 ± 0.011 OD); Ferric chloride (1.682 ± 0.022 OD); Glutamine (2.136 ± 0.067 OD); polyethyleneglycol (0.873 ± 0.041 OD); 2.5% NaCl (1.138 ± 0.023 OD); Neem oil (1.699 ± 0.176 OD); inoculum concentration 2.00% (1.625 ± 0.007 OD); The maximum EPS production was viewed at the 72hrs of incubation at static condition (1.58 ± 0.122 OD).