DNA binding and cytotoxicity of newly synthesized schiff base (z)-4-(((2-hydroxy phenyl) amino) (phenyl) methylene)-3-methyl-1-phenyl-1hpyrazol-5(4H)-one and its analogues.

ABSTRACT

A novel Schiff base (Z)-4-(((2-hydroxy phenyl)amino)(phenyl)methylene)-3-methyl-1-phenyl-1Hpyrazol- 5(4H)-one and its analogues were synthesized by condensation of 5-methyl-2-phenyl-4-substituted pyrazolin- 3-one with 2-amino phenol from alcoholic solution.Schiff base ligands arecharacterizedby IR, UV, NMR, elemental analysis and single crystal X- ray diffraction analysis. These potential ligands are subjected to DNA binding analysis against the Calf thymus DNA, their binding constant values were calculated and compared to the standard ruthenium intercalators. The cytotoxic natureof these Schiff base ligands was also studied with the human breast cancer cell line MCF-7 and their IC50 values were determined.